EWOD-driven droplet microfluidic device integrated with optoelectronic tweezers as an automated platform for cellular isolation and analysis

被引：122

作者：

Shah, Gaurav J. ^{[1
]}

Ohta, Aaron T. ^{[2
]}

Chiou, Eric P. -Y. ^{[1
]}

Wu, Ming C. ^{[3
]}

Kim, Chang-Jin ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Los Angeles, CA 90024 USA

[2] Univ Hawaii, Honolulu, HI 96822 USA

[3] Univ Calif Berkeley, Berkeley, CA 94720 USA

来源：

LAB ON A CHIP | 2009年 / 9卷 / 12期

关键词：

ON-A-CHIP; ELECTROWETTING-BASED ACTUATION; DIGITAL MICROFLUIDICS; LIQUID DROPLETS; SEPARATION; CELLS; DIELECTROPHORESIS; MANIPULATION; PARTICLES; FORCES;

D O I：

10.1039/b821508a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the integration of two technologies: droplet microfluidics using electrowetting-on-dielectric (EWOD) and individual particle manipulation using optoelectronic tweezers (OET)-in one microfluidic device. The integrated device successfully demonstrates a sequence involving both EWOD and OET operations. We encountered various challenges during integration of the two different technologies and present how they are addressed. To show the applicability of the device in cellular biology, live HeLa cells are used in the experiments. The unique advantages of EWOD and OET make their integration a significant step towards a powerful tool for many applications, such as single cell studies involving multiplexed environmental stimuli.

引用

页码：1732 / 1739

页数：8

共 58 条

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