Stability of herpes simplex virus DNA in cerebrospinal fluid specimens

被引：11

作者：

Wiedbrauk, DL

Cunningham, W

机构：

[1] Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, MI

[2] Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, MI 48073-6769

来源：

DIAGNOSTIC MOLECULAR PATHOLOGY | 1996年 / 5卷 / 04期

关键词：

herpes simplex virus; polymerase chain reaction; DNA stability; degradation; encephalitis;

D O I：

10.1097/00019606-199612000-00004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polymerase chain reaction methods are becoming the standard for the diagnosis of herpes simplex virus (HSV) encephalitis. Little is known, however, about the stability of HSV DNA in cerebrospinal fluid (CSF) specimens. Our results demonstrate that HSV DNA is extremely stable in CSF specimens containing lymphocytes and monocytes. We observed little DNA degradation in specimens stored for 30 days at room temperature (20-23 degrees C), refrigerator temperature (2-8 degrees C), or freezer temperatures (-18 to -22 degrees C and -69 to -72 degrees C). Specimen storage conditions are therefore not so critical for HSV encephalitis detection as for other viral illnesses. It is therefore not necessary to perform a second lumbar puncture to obtain a fresh specimen for the detection of HSV DNA.

引用

页码：249 / 252

页数：4

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