CrAsH: a biarsenical multi-use affinity probe with low non-specific fluorescence

被引:41
作者
Cao, Haishi [1 ]
Chen, Baowei [1 ]
Squier, Thomas C. [1 ]
Mayer, M. Uljana [1 ]
机构
[1] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA
关键词
D O I
10.1039/b602699k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
CrAsH is a tetracysteine-binding probe which has improved properties in terms of signal-to-noise ratio and pH dependence of fluorescence compared to the parent compound.
引用
收藏
页码:2601 / 2603
页数:3
相关论文
共 20 条
[1]   New biarsenical Ligands and tetracysteine motifs for protein labeling in vitro and in vivo: Synthesis and biological applications [J].
Adams, SR ;
Campbell, RE ;
Gross, LA ;
Martin, BR ;
Walkup, GK ;
Yao, Y ;
Llopis, J ;
Tsien, RY .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (21) :6063-6076
[2]   Short tetracysteine tags to β-tubulin demonstrate the significance of small labels for live cell imaging [J].
Andresen, M ;
Schmitz-Salue, R ;
Jakobs, S .
MOLECULAR BIOLOGY OF THE CELL, 2004, 15 (12) :5616-5622
[3]   CHARACTERIZATION OF UPTAKE AND HYDROLYSIS OF FLUORESCEIN DIACETATE AND CARBOXYFLUORESCEIN DIACETATE BY INTRACELLULAR ESTERASES IN SACCHAROMYCES-CEREVISIAE, WHICH RESULT IN ACCUMULATION OF FLUORESCENT PRODUCT [J].
BREEUWER, P ;
DROCOURT, JL ;
BUNSCHOTEN, N ;
ZWIETERING, MH ;
ROMBOUTS, FM ;
ABEE, T .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (04) :1614-1619
[4]   Structural uncoupling between opposing domains of oxidized calmodulin underlies the enhanced binding affinity and inhibition of the plasma membrane Ca-ATPase [J].
Chen, BW ;
Mayer, MU ;
Squier, TC .
BIOCHEMISTRY, 2005, 44 (12) :4737-4747
[5]   Recent developments in the analysis of protein complexes [J].
Dziembowski, A ;
Séraphin, B .
FEBS LETTERS, 2004, 556 (1-3) :1-6
[6]   Novel approaches to map protein interactions [J].
Figeys, D .
CURRENT OPINION IN BIOTECHNOLOGY, 2003, 14 (01) :119-125
[7]   Multicolor and electron microscopic imaging of connexin trafficking [J].
Gaietta, G ;
Deerinck, TJ ;
Adams, SR ;
Bouwer, J ;
Tour, O ;
Laird, DW ;
Sosinsky, GE ;
Tsien, RY ;
Ellisman, MH .
SCIENCE, 2002, 296 (5567) :503-507
[8]  
Griffin BA, 2000, METHOD ENZYMOL, V327, P565
[9]   Specific covalent labeling of recombinant protein molecules inside live cells [J].
Griffin, BA ;
Adams, SR ;
Tsien, RY .
SCIENCE, 1998, 281 (5374) :269-272
[10]   Manipulating proteins with chemistry: a cross-section of chemical biology [J].
Hahn, ME ;
Muir, TW .
TRENDS IN BIOCHEMICAL SCIENCES, 2005, 30 (01) :26-34