Preparation of immobilized cellulase beads and their application to hydrolysis of cellulosic materials

被引：23

作者：

Kumakura, M

机构：

[1] Department of Bioscience, Teikyo Univ. of Sci. and Technology, KiaTsuru, Yamanashi 409-01

来源：

PROCESS BIOCHEMISTRY | 1997年 / 32卷 / 07期

关键词：

D O I：

10.1016/S0032-9592(97)00011-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cellulase was immobilized on aluminium oxide beads with a polymer matrix by radiation polymerization at low temperature using various monomers. The optimum conditions for the preparation of the immobilized enzyme beads were obtained, and the characteristics of the immobilized enzyme beads investigated by repeated batch enzyme reactions. The cellulose component of chaff, pretreated by electron beam irradiation and subsequent pulverization, was effectively hydrolysed by the immobilized enzyme beads, in which the conversion (hydrolysis) yield in the repeated batch enzyme reactions with 10% chaff slurry was 53%. (C) 1997 Elsevier Science Ltd.

引用

页码：555 / 559

页数：5

共 12 条

[1] INTERGENERIC HYBRIDIZATION OF TRICHODERMA-REESEI QM9414 AND SACCHAROMYCES-CEREVISIAE NCIM-3288 BY PROTOPLAST FUSION [J].