Development of the electrochemical biosensor for organophosphate chemicals using CNT/ionic liquid bucky gel electrode

被引：56

作者：

Choi, Bong Gill ^{[1
]}

Park, HoSeok ^{[1
]}

Park, Tae Jung ^{[2
,3
]}

Kim, Dong Hyun ^{[1
]}

Lee, Sang Yup ^{[1
,2
,3
,4
,5
]}

Hong, Won Hi ^{[1
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, BK21 Program, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, Inst BioCentury, Taejon 305701, South Korea

[3] Korea Adv Inst Sci & Technol, Ctr Syst & Synthet Biotechnol, BioProc Engn Res Ctr, Taejon 305701, South Korea

[4] Korea Adv Inst Sci & Technol, Dept Biol Sci, Dept Bio & Brain Engn, Taejon 305701, South Korea

[5] Korea Adv Inst Sci & Technol, Bioinformat Res Ctr, Taejon 305701, South Korea

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2009年 / 11卷 / 03期

关键词：

Biosensor; Organophosphorus hydrolase; Ionic liquid; CNT; Electrochemical property; CARBON NANOTUBES; IONIC LIQUIDS; PESTICIDES; HYDROLASE; BIOELECTROCHEMISTRY;

D O I：

10.1016/j.elecom.2009.01.006

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Organophosphorus hydrolase (OPH) immobilized on CNT/ionic liquid (IL) electrodes were prepared by using three different intrinsic kinds of ILs, as binders. CNTs/lLs lead to dramatic electrochemical enhancements with respect to response time, stability, and sensitivity of composite electrodes. In addition, the electrochemical and biocatalytic properties of three-composite electrodes were strongly influenced by different types of ILs used, as verified by cyclic voltammetry and chronoamperometry. These results were attributed to the conformational changes of the microenvironment between the OPH and the composite electrodes within three different types of ILs. In particular, the biocatalytic signals of three OPH/CNT/ILs-modified electrodes increased linearly to the concentration of paraoxon in a wide range of 2-20 mu M These findings provide a deep understanding of the role of each IL on the modified electrodes, enabling to enhance electrochemical properties for biosensors. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：672 / 675

页数：4

共 13 条

[1] The dramatic acceleration effect of imidazolium ionic liquids on electron transfer reactions [J].