Bni5p, a septin-interacting protein, is required for normal septin function and cytokinesis in Saccharomyces cerevisiae

被引:65
作者
Lee, PR
Song, S
Ro, HS
Park, CJ
Lippincott, J
Li, R
Pringle, JR
De Virgilio, C
Longtine, MS
Lee, KS
机构
[1] NCI, Lab Metab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA
[2] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA
[3] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
[4] Univ N Carolina, Program Mol Biol & Biotechnol, Chapel Hill, NC 27599 USA
[5] Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74078 USA
关键词
D O I
10.1128/MCB.22.19.6906-6920.2002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the budding yeast Saccharomyces cerevisiae, the Cdc3p, Cdc10p, Cdc11p, Cdc12p, and Sep7p/Shs1p septins assemble early in the cell cycle in a ring that marks the future cytokinetic site. The septins appear to be major structural components of a set of filaments at the mother-bud neck and function as a scaffold for recruiting proteins involved in cytokinesis and other processes. We isolated a novel gene, BN15, as a dosage suppressor of the cdc12-6 growth defect. Overexpression of BN15 also suppressed the growth defects of cdc10-1, cdc11-6, and sep70 strains. Loss of BN15 resulted in a cytokinesis defect, as evidenced by the formation of connected cells with shared cytoplasms, and deletion of BN15 in a cdc3-6, cde10-1, cdc11-6, cdc12-6, or sep70 mutant strain resulted in enhanced defects in septin localization and cytokinesis. Bni5p localizes to the mother-bud neck in a septin-dependent manner shortly after bud emergence and disappears from the neck approximately 2 to 3 min before spindle disassembly. Two-hybrid, in vitro binding, and protein-localization studies suggest that Bni5p interacts with the N-terminal domain of Cdc11p, which also appears to be sufficient for the localization of Cdc11p, its interaction with other septins, and other critical aspects of its function. Our data suggest that the Bni5p-septin interaction is important for septin ring stability and function, which is in turn critical for normal cytokinesis.
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页码:6906 / 6920
页数:15
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