Characterization of the piRNA complex from rat testes

被引：757

作者：

Lau, Nelson C.

Seto, Anita G.

Kim, Jinkuk

Kuramochi-Miyagawa, Satomi

Nakano, Toru

Bartel, David P.

Kingston, Robert E.

机构：

[1] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA

[2] MIT, Harvard MIT Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[3] MIT, Howard Hughes Med Inst, Cambridge, MA 02142 USA

[4] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[5] Osaka Univ, Microbial Dis Res Inst, Dept Mol Cell Biol, Suita, Osaka 5650871, Japan

[6] MIT, Dept Biol, Cambridge, MA 02139 USA

来源：

SCIENCE | 2006年 / 313卷 / 5785期

关键词：

D O I：

10.1126/science.1130164

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Small noncoding RNAs regulate processes essential for cell growth and development, including mRNA degradation, translational repression, and transcriptional gene silencing (TGS). During a search for candidate mammalian factors for TGS, we purified a complex that contains small RNAs and Riwi, the rat homolog to human Piwi. The RNAs, frequently 29 to 30 nucleotides in length, are called Piwi-interacting RNAs (piRNAs), 94% of which map to 100 defined (<= 101 kb) genomic regions. Within these regions, the piRNAs generally distribute across only one genomic strand or distribute on two strands but in a divergent, nonoverlapping manner. Preparations of piRNA complex ( piRC) contain rRecQ1, which is homologous to qde-3 from Neurospora, a gene implicated in silencing pathways. Piwi has been genetically linked to TGS in flies, and slicer activity cofractionates with the purified complex. These results are consistent with a gene-silencing role for piRC in mammals.

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页码：363 / 367

页数：5

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