Nrf2 activity is lost in the spinal cord and its astrocytes of aged mice

被引：66

作者：

Duan, Weisong ^{[2
]}

Zhang, Ruiyan ^{[2
]}

Guo, Yansu ^{[1
,2
]}

Jiang, Yifang ^{[2
]}

Huang, Yanli ^{[2
]}

Jiang, Hong ^{[2
]}

Li, Chunyan ^{[1
,2
]}

机构：

[1] Inst Cardiocerebrovasc Dis, Shijiazhuang 050000, Hebei, Peoples R China

[2] Hebei Med Univ, Hosp 2, Dept Neurol, Shijiazhuang 050000, Hebei, Peoples R China

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 2009年 / 45卷 / 07期

关键词：

Aging; Astrocytes; Oxidative stress; NQO1; Nrf2; OXIDATIVE STRESS; GLUTAMATE; PROTEIN; SENSITIVITY; EXPRESSION; COMPONENTS; KNOCKOUT; INCREASE; GENES;

D O I：

10.1007/s11626-009-9194-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We report herein a study of aging using in vitro and in vivo models. Glial fibrillary acidic protein and ferritin expression levels increased, and the levels of glutamate transporter 1 and transferrin receptor 1 decreased in aging mouse spinal cord and its astrocytes. Mitochondrial transmembrane potential in astrocytes decreased after 60 d of culture. Given the relationship between aging and loss of antioxidant tolerance capacity, we examined the expression of heme oxygenase 1 (HO1) and NAD(P)H/quinone oxidoreductase 1 (NQO1) in the old mouse astrocytes and spinal cord. Indeed, both antioxidant enzymes decreased there. Total nuclear factor E2-related factor 2, which governs basal and inducible expression of HO1 and NQO1, decreased significantly. Significantly, epigallocatechin gallate restored the Nrf2 activity.

引用

页码：388 / 397

页数：10

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