Induction of zona radiata and vitellogenin genes in estradiol and nonylphenol exposed male sheepshead minnows (Cyprinodon variegatus)

被引：31

作者：

Knoebl, I

Hemmer, MJ

Denslow, ND ^{[1
]}

机构：

[1] Univ Florida, Interdisciplinary Ctr Biotechnol Res, Gainesville, FL 32610 USA

[2] US EPA, Gulf Ecol Div, Gulf Breeze, FL 32561 USA

[3] Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USA

来源：

MARINE ENVIRONMENTAL RESEARCH | 2004年 / 58卷 / 2-5期

关键词：

vitellogenin; zona radiata protein; estradiol; nonylphenol; real time PCR;

D O I：

10.1016/j.marenvres.2004.03.043

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Several genes normally induced by estradiol (E-2) in female fish, those for vitellogenins (VTGs) and zona radiata proteins (ZRPs), are also inducible in males exposed to estrogenic chemicals. Male sheepshead minnows (SHM) were exposed to both E-2 and para-nonylphenol (NP), at several doses and times to determine a dose-response. Quantitative real time PCR was used to measure mRNA for VTG1, VTG2, ZRP2 and ZRP3. Both E-2 and NP elicited a dose-response increase in all of the mRNAs tested. Exposure to both chemicals resulted in VTG2 expression at about a 10-fold lower level than VTG1, and ZRP2 expression at a lower level than ZRP3. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：547 / 551

页数：5