Isolation and characterization of an aspartic proteinase gene from cowpea (Vigna unguiculata L. Walp.)

被引：7

作者：

de Carvalho, MHC

Pham-Thi, AT

Gareil, M

d'Arcy-Lameta, A

Fodil, YZ

机构：

[1] Univ Paris 12, UMR IRD 137 Biosol, Lab Ecophysiol Mol, F-94010 Creteil, France

[2] Univ Nova Lisboa, ITQB, Lab Ecofisiol Mol, P-2781901 Oeiras, Portugal

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2004年 / 161卷 / 08期

关键词：

aspartic proteinase; cowpea; genomic library screening; expression analysis; senescence;

D O I：

10.1016/j.jplph.2003.12.007

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A cowpea (Vigna unguiculata cv. EPACE-1) aspartic proteinase (AP) gene was isolated by genomic library screening. Sequence analysis shows that this AP gene follows the same pattern of intron/exon number and organization as the other isolated plant AP genes, which are distinct from other solved AP genes. Northern blot analysis revealed that cowpea AP accumulates in leaves and stems but not in roots, indicating tissue-specific expression. An increased accumulation of transcripts during senescence suggests enzyme involvement in this process. (C) 2004 Elsevier GmbH. All rights reserved.

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页码：971 / 976

页数：6

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