A Near-Infrared Neutral pH Fluorescent Probe for Monitoring Minor pH Changes: Imaging in Living HepG2 and HL-7702 Cells

被引：460

作者：

Tang, Bo ^{[1
]}

Yu, Fabiao ^{[1
]}

Li, Ping ^{[1
]}

Tong, Lili ^{[1
]}

Duan, Xia ^{[1
]}

Xie, Ting ^{[1
]}

Wang, Xu ^{[1
]}

机构：

[1] Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Engn Res Ctr Pesticide & Med Intermediate Clean P, Key Lab Mol & Nano Probes,Minist Educ, Jinan 250014, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 08期

基金：

国家杰出青年科学基金; 国家自然科学基金重大项目; 中国国家自然科学基金;

关键词：

BORDER NA+/H+ EXCHANGER; INTRACELLULAR PH; PHOTOPHYSICAL PROPERTIES; AQUEOUS-SOLUTIONS; DUAL-EXCITATION; EMISSION; ACIDIFICATION; INDICATOR; APOPTOSIS; VESICLES;

D O I：

10.1021/ja809149g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A near-neutral pH near-infrared (NIR) fluorescent probe utilizing a fluorophore-spacer-receptor molecular framework that can modulate the fluorescence emission intensity through a fast photoinduced electron-transfer process was developed. Our strategy was to choose tricarbocyanine (Cy), a NIR fluorescent dye with high extinction coefficients, as a fluorophore, and 4'-(aminomethylphenyl)-2,2':6',2 ''-terpyridine (Tpy) as a receptor. The pH titration indicated that Tpy-Cy can monitor the minor physiological pH fluctuations with a pK(a) of similar to 7.10 near physiological pH, which is valuable for intracellular pH researches. The probe responds linearly and rapidly to minor pH fluctuations within the range of 6.70-7.90 and exhibits strong dependence on pH changes. As expected, the real-time imaging of cellular pH and the detection of pH in situ was achieved successfully in living HepG2 and HL-7702 cells by this probe. It is shown that the probe effectively avoids the influence of autofluorescence and native cellular species in biological systems and meanwhile exhibits high sensitivity, good photostability, and excellent cell membrane permeability.

引用

页码：3016 / 3023

页数：8