Saturating irradiance-induced photoinhibition without monomerisation of photosystem 2 dimer in soybean leaves

被引：3

作者：

Cai, SQ ^{[1
]}

Chen, GY ^{[1
]}

Zhang, HB ^{[1
]}

Xu, DQ ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China

来源：

PHOTOSYNTHETICA | 2002年 / 40卷 / 02期

关键词：

dimer; Glycine max; light-harvesting complex 2; monomerisation; photosystem; 2; proteins;

D O I：

10.1023/A:1021341623339

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The oligomeric state of photosystem 2 (PS2) complex in soybean leaves treated with saturating irradiance was studied by non-denaturing polyacrylamide gel electrophoresis (PAGE) and gel filtration chromatography. PS2 dimers resolved by non-denaturing PAGE accounted for about 75 % of total PS2 complex and there was no significant difference in the ratio of PS2 dimer to monomer between samples from saturating irradiance-treated and fully dark-adapted leaves. Furthermore, BBY particles were resolved into four chlorophyll-enriched fractions by gel filtration chromatography. From their molecular masses and protein components, these fractions were deduced to be PS2 dimer, PS2 monomer, oligomeric light-harvesting complex 2 (LHC2), and monomeric LHC2. Also, no change in the proportion of PS2 dimer in total PS2 was observed in the granal region of thylakoid membranes from soybean leaves after saturating irradiation. Hence the dimer is the predominant natural form of PS2 in vivo and no monomerisation of PS2 dimer occurs during saturating irradiance-induced photoinhibition in soybean leaves.

引用

页码：215 / 220

页数：6

共 28 条

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