Distribution and variation of bacitracin synthetase gene sequences in laboratory stock strains of Bacillus licheniformis

被引：42

作者：

Ishihara, H ^{[1
]}

Takoh, M ^{[1
]}

Nishibayashi, R ^{[1
]}

Sato, A ^{[1
]}

机构：

[1] Tohoku Gakuin Univ, Fac Liberal Arts, Izumi Ku, Sendai, Miyagi 9813193, Japan

来源：

CURRENT MICROBIOLOGY | 2002年 / 45卷 / 01期

关键词：

D O I：

10.1007/s00284-001-0041-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Distribution and variation of bacitracin synthetase gene (bac) sequences in 22 laboratory stock strains of Bacillus licheniformis were studied by Southern hybridization of bac gene probes from B. licheniformis ATCC 10716 to genomic PstI or Hind111 restriction fragments. Eleven Strains gave hybridization signals. These hybridization patterns were classed into two types, Eight strains Showed similar patterns to that of ATCC 10716 and all, except one, produced bacitracin. The three Strains showed fairly different hybridization patterns from that of ATCC 10716, and one (ATCC 33632) of them produced bacitracin. None of the remaining 11 strains. including ATCC 14580 (type strain), gave any hybridization signals, All strains carrying bac gene sequences were erythromycin resistant. With one exception. all strains without bac gene sequences were erythromycin sensitive. These results show that B. licheniformis strains are divided into two groups with respect to presence of bac gene sequences and erythromycin resistance.

引用

页码：18 / 23

页数：6

共 23 条

[1]

ARRIAGADA A, 1949, BRIT J EXP PATHOL, V30, P425

[2] BACITRACIN PRODUCTION BY A NEW STRAIN OF BACILLUS-SUBTILIS - EXTRACTION, PURIFICATION, AND CHARACTERIZATION [J].

AZEVEDO, EC ;

RIOS, EM ;

FUKUSHIMA, K ;

CAMPOSTAKAKI, GM .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1993, 42 (01) :1-9

[3]

Berdy J, 1974, Adv Appl Microbiol, V18, P309, DOI 10.1016/S0065-2164(08)70573-2

[4] PCR fingerprinting of whole genomes:: the spacers between the 16S and 23S rRNA genes and of intergenic tRNA gene regions reveal a different intraspecific genomic variability of Bacillus cereus and Bacillus licheniformis [J].

Daffonchio, D ;

Borin, S ;

Frova, G ;

Manachini, PL ;

Sorlini, C .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 :107-116

[5]

DUNCAN KE, 1994, EVOLUTION, V48, P2002, DOI 10.1111/j.1558-5646.1994.tb02229.x

[6]

FROYSHOV O, 1984, BIOTECHNOLOGY IND AN, P665

[7]

GORDON RE, 1973, AGR HDB USDA, V427, P34

[8]

GRIMONT F, 1991, NUCL ACID TECHNIQUES, P249

[9]

HAAVIK HI, 1975, ACTA PATH MICRO IM B, V83, P534

[10] MOLECULAR-CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE BACILLUS-LICHENIFORMIS BACITRACIN SYNTHETASE-2 GENE [J].

ISHIHARA, H ;

HARA, N ;

IWABUCHI, T .

JOURNAL OF BACTERIOLOGY, 1989, 171 (03) :1705-1711

← 1 2 3 →