Isolation and characterization of luffacylin, a ribosome inactivating peptide with anti-fungal activity from sponge gourd (Luffa cylindrica) seeds

被引：87

作者：

Parkash, A ^{[1
]}

Ng, TB ^{[1
]}

Tso, WW ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Fac Med, Dept Biochem, Shatin, Hong Kong, Peoples R China

来源：

PEPTIDES | 2002年 / 23卷 / 06期

关键词：

sponge gourd; seeds; anti-fungal; ribosome inactivation;

D O I：

10.1016/S0196-9781(02)00045-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A purification scheme involving ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and ion exchange chromatography on CM-Sepharose and Mono S was employed to isolate a peptide with a molecular weight of 7.8 kDa from sponge gourd seeds. The peptide, which was designated luffacylin, exhibited an N-terminal sequence with pronounced resemblance to that of the 6.5 kDa arginine-glutamate rich polypeptide previously isolated from sponge gourd seeds. Luffacylin inhibited translation in a rabbit reticulocyte lysate system with an IC50 of 140 pM and reacted positively in the N-glycosidase assay for ribosome inactivating proteins. Luffacylin exerted anti-fungal activity against Mycosphaerella arachidicola and Fusarium oxysporum. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：1019 / 1024

页数：6

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