Synthesis and biological activity of polyethylene glycol-mouse nerve growth factor conjugate

被引：23

作者：

Belcheva, N ^{[1
]}

Woodrow-Mumford, K ^{[1
]}

Mahoney, MJ ^{[1
]}

Saltzman, WM ^{[1
]}

机构：

[1] Cornell Univ, Sch Chem Engn, Ithaca, NY 14853 USA

来源：

BIOCONJUGATE CHEMISTRY | 1999年 / 10卷 / 06期

关键词：

D O I：

10.1021/bc990001k

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Conjugation of poly(ethylene glycol) derivatives with therapeutic proteins is a promising approach for enhancing protein stability and, therefore, effectiveness. An N-hydroxysuccinimidyl ester of fluorescein-PEG 2000 was used for chemical modification of mouse nerve growth factor (mNGF), a dimeric protein with therapeutic potential for Alzheimer's disease. The mNGF-PEG2000-fluorescein conjugate was characterized by RP-HPLC, spectrofluorometry, and SDS-PAGE and was biologically active, as determined by two independent NGF-specific assays (enhancement of ChAT activity in fetal neurons and neurite outgrowth in PC12 cells). The conjugate was not detectable by a standard NGF ELISA, suggesting a fortuitous reduction in protein recognition by antibodies.

引用

页码：932 / 937

页数：6

共 25 条

[1]

Backman C, 1996, J NEUROSCI, V16, P5437

[2]

Atrophy of cholinergic basal forebrain neurons following excitotoxic cortical lesions is reversed by intravenous administration of an NGF conjugate [J].