Gateway-based destination vectors for functional analyses of bacterial ORFeomes:: Application to the min system in Brucella abortus

被引：37

作者：

Hallez, Regis ^{[1
]}

Letesson, Jean-Jacques ^{[1
]}

Vandenhaute, Jean ^{[1
]}

De Bolle, Xavier ^{[1
]}

机构：

[1] Univ Namur, Unite Rech Biol Mol, B-5000 Namur, Belgium

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 04期

关键词：

D O I：

10.1128/AEM.01873-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.

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页码：1375 / 1379

页数：5

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