ORFeome cloning and systems biology: Standardized mass production of the parts from the parts-list

被引：53

作者：

Brasch, MA

Hartley, JL

Vidal, M ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Ctr Canc Syst Biol, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

[4] Atto Biosci, Rockville, MD 20850 USA

[5] NCI, SAIC, Frederick, MD 21702 USA

来源：

GENOME RESEARCH | 2004年 / 14卷 / 10B期

关键词：

D O I：

10.1101/gr.2769804

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Together with metabolites, proteins and RNAs form complex biological systems through highly intricate networks of physical and functional interactions. Large-scale studies aimed at a molecular understanding of the structure, function, and dynamics of proteins and RNAs in the context of cellular networks require novel approaches and technologies. This Special Issue of Genome Research features strategies for the high-throughput construction and manipulation of complete sets of protein-encoding open reading frames (ORFeome), gene promoters (promoterome), and noncoding RNAs, as predicted from genome and transcriptome sequences. Here we discuss the use of a recombinational cloning system that allows efficiency, adaptability, and compatibility in the generation of ORFeome, promoterome, and other resources. © 2004 by Cold Spring Harbor Laboratory Press.

引用

页码：2001 / 2009

页数：9

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