NPY in rat retina is present in neurons, in endothelial cells and also in microglial and Muller cells

被引：27

作者：

Alvaro, Ana Rita

Rosmaninho-Salgado, Joana

Santiago, Ana Raquel

Martins, Joao

Aveleira, Celia

Santos, Paulo F.

Pereira, Tiago

Gouveia, Denisa

Carvalho, Ana Luisa

Grouzmann, Eric

Ambrosio, Antonio Francisco

Cavadas, Claudia ^{[1
]}

机构：

[1] Univ Coimbra, Fac Pharm, IBILI, Ctr Ophthalmol, P-3020123 Coimbra, Portugal

[2] Univ Coimbra, Ctr Neurosci & Cell Biol, P-3020123 Coimbra, Portugal

[3] CHU Vaudois, Div Clin Pharmacol & Toxicol, CH-1011 Lausanne, Switzerland

来源：

NEUROCHEMISTRY INTERNATIONAL | 2007年 / 50卷 / 05期

关键词：

neuropeptide Y (NPY); rat retina; microglial cells; Muller cells; endothelial cells; NPY receptors; NPY release;

D O I：

10.1016/j.neuint.2007.01.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

NPY is present in the retina of different species but its role is not elucidated yet. In this work, using different rat retina in vitro models (whole retina, retinal cells in culture, microglial cell cultures, rat Muller cell line and retina endothelial cell line), we demonstrated that NPY staining is present in the retina in different cell types: neurons, macroglial, microglial and endothelial cells. Retinal cells in culture express NPY Y-1, Y-2, Y-4 and Y-5 receptors. Retina endothelial cells express all NPY receptors except NPY Y-5 receptor. Moreover, NPY is released from retinal cells in culture upon depolarization. In this study we showed for the first time that NPY is present in rat retina microglial cells and also in rat Muller cells. These in vitro models may open new perspectives to study the physiology and the potential pathophysiological role of NPY in the retina. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：757 / 763

页数：7

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