Zizimin1, a novel Cdc42 activator, reveals a new GEF domain for Rho proteins

被引：142

作者：

Meller, N ^{[1
]}

Irani-Tehrani, M ^{[1
]}

Kiosses, WB ^{[1
]}

Del Pozo, MA ^{[1
]}

Schwartz, MA ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, Div Vasc Biol, La Jolla, CA 92037 USA

来源：

NATURE CELL BIOLOGY | 2002年 / 4卷 / 09期

关键词：

D O I：

10.1038/ncb835

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The Rho family GTPases Rac, Rho and Cdc42 are critical in regulating the actin-based cytoskeleton, cell migration, growth, survival and gene expression. These GTPases are activated by guanine nucleotide-exchange factors (GEFs). A biochemical search for Cdc42 activators led to the cloning of zizimin1, a new protein whose overexpression induces Cdc42 activation. Sequence comparison combined with mutational analysis identified a new domain, which we named CZH2, that mediates direct interaction with Cdc42. CZH2-containing proteins constitute a new superfamily that includes the so-called 'CDM' proteins that bind to and activate Rac. Together, the results suggest that CZH2 is a new GEF domain for the Rho family of proteins.

引用

页码：639 / 647

页数：9

共 29 条

[1] Effectors for the Rho GTPases [J].

Aspenström, P .

CURRENT OPINION IN CELL BIOLOGY, 1999, 11 (01) :95-102

[2]

Bateman A, 2004, NUCLEIC ACIDS RES, V32, pD138, DOI [10.1093/nar/gkp985, 10.1093/nar/gkh121, 10.1093/nar/gkr1065]

[3] PREDICTING COILED COILS BY USE SF PAIRWISE RESIDUE CORRELATIONS [J].

BERGER, B ;

WILSON, DB ;

WOLF, E ;

TONCHEV, T ;

MILLA, M ;

KIM, PS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (18) :8259-8263

[4] Rho GTPases and their effector proteins [J].

Bishop, AL ;

Hall, A .

BIOCHEMICAL JOURNAL, 2000, 348 (02) :241-255

[5] The Dbl family of oncogenes [J].

Cerione, RA ;

Zheng, Y .

CURRENT OPINION IN CELL BIOLOGY, 1996, 8 (02) :216-222

[6] GEFs: structural basis for their activation of small GTP-binding proteins [J].

Cherfils, J ;

Chardin, P .

TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (08) :306-311

[7] MULTIPLE SEQUENCE ALIGNMENT WITH HIERARCHICAL-CLUSTERING [J].

CORPET, F .

NUCLEIC ACIDS RESEARCH, 1988, 16 (22) :10881-10890

[8] Adhesion to the extracellular matrix regulates the coupling of the small GTPase Rac to its effector PAK [J].

del Pozo, MA ;

Price, LS ;

Alderson, NB ;

Ren, XD ;

Schwartz, MA .

EMBO JOURNAL, 2000, 19 (09) :2008-2014

[9]

del Pozo MA, 1999, EUR J IMMUNOL, V29, P3609

[10] Haematopoietic cell-specific CDM family protein DOCK2 is essential for lymphocyte migration [J].

Fukui, Y ;

Hashimoto, O ;

Sanui, T ;

Oono, T ;

Koga, H ;

Abe, M ;

Inayoshi, A ;

Noda, M ;

Oike, M ;

Shirai, T ;

Sasazuki, T .

NATURE, 2001, 412 (6849) :826-831

← 1 2 3 →