Colorimetric Assay for Urinary Track Infection Disease Diagnostic on Flexible Substrate

被引：4

作者：

Safavieh, Mohammadali ^{[1
]}

Ahmed, Minhaz Uddin ^{[1
]}

Zourob, Mohammed ^{[1
]}

机构：

[1] Univ Quebec, Inst Natl Rech Sci, Energie Mat Telecommun INRS EMT, Varennes, PQ J3X 1S2, Canada

来源：

PHOTONICS NORTH 2012 | 2012年 / 8412卷

关键词：

Point of care; diagnostic device; Loop-mediated isothermal amplification; Lab on a chip; colorimetric assay; Nucleic acid; E.coli detection; ESCHERICHIA-COLI;

D O I：

10.1117/12.2001487

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We are presenting cassette as a novel point of care diagnostic device. This device is easy to use, low cost to prepare, high throughput and can analyze several samples at the same time. We first, demonstrate the preparation method of the device. Then, fabrication of the flexible substrate has been presented. The device has been used for detection of the real sample of E.coli bacteria following by colorimetric detection. We have shown that we could detect 30 cfu/ml bacteria and 100 fg/mu l of Staphylococous aureus DNA in 1 hr using LAMP amplification technique. This device will be helpful in hospitals and doctor's office for analysis of several patients' samples at the same time.

引用

页数：6

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