Quantitative phase tomography of Arabidopsis seeds reveals intercellular void network

被引:149
作者
Cloetens, Peter
Mache, Regis
Schlenker, Michel
Lerbs-Mache, Silva
机构
[1] Univ Grenoble 1, Lab Plastes & Differenciat Cellulaire, CNRS, F-38041 Grenoble 9, France
[2] European Synchrotron Radiat Facil, F-38043 Grenoble, France
[3] Univ Grenoble 1, Lab Louis Neel, CNRS, Inst Natl Polytech Grenoble, F-38042 Grenoble, France
关键词
mature seed structure; synchrotron radiation; x-ray imaging; embryo organs; cell components;
D O I
10.1073/pnas.0603490103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We used quantitative phase tomography with synchrotron radiation to elucidate the 3D structure of Arabidopsis seeds in their native state. The cells are clearly distinguished, and their internal structure is revealed through local variations in electron density. We visualized a 3D network of intercellular air space that might allow immediate gas exchange for energy supply during germination and/or serve for rapid water uptake and distribution during imbibition.
引用
收藏
页码:14626 / 14630
页数:5
相关论文
共 28 条
  • [21] Computed tomography of cryogenic cells
    Schneider, G
    Anderson, E
    Vogt, S
    Knöchel, C
    Weiss, D
    Legros, M
    Larabell, C
    [J]. SURFACE REVIEW AND LETTERS, 2002, 9 (01) : 177 - 183
  • [22] Biological imaging by soft x-ray diffraction microscopy
    Shapiro, D
    Thibault, P
    Beetz, T
    Elser, V
    Howells, M
    Jacobsen, C
    Kirz, J
    Lima, E
    Miao, H
    Neiman, AM
    Sayre, D
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (43) : 15343 - 15346
  • [23] On the possibilities of x-ray phase contrast microimaging by coherent high-energy synchrotron radiation
    Snigirev, A
    Snigireva, I
    Kohn, V
    Kuznetsov, S
    Schelokov, I
    [J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 1995, 66 (12) : 5486 - 5492
  • [24] Molecular physiology of legume seed development
    Weber, H
    Borisjuk, L
    Wobus, U
    [J]. ANNUAL REVIEW OF PLANT BIOLOGY, 2005, 56 : 253 - 279
  • [25] Phase-contrast imaging using polychromatic hard X-rays
    Wilkins, SW
    Gureyev, TE
    Gao, D
    Pogany, A
    Stevenson, AW
    [J]. NATURE, 1996, 384 (6607) : 335 - 338
  • [26] Pectin: cell biology and prospects for functional analysis
    Willats, WGT
    McCartney, L
    Mackie, W
    Knox, JP
    [J]. PLANT MOLECULAR BIOLOGY, 2001, 47 (1-2) : 9 - 27
  • [27] YE ZH, 1991, PLANT J, V1, P175, DOI 10.1111/j.1365-313X.1991.00175.x
  • [28] Optimization of phase contrast imaging using hard x rays
    Zabler, S
    Cloetens, P
    Guigay, JP
    Baruchel, J
    Schlenker, M
    [J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 2005, 76 (07)