Selection of migration parameters for a highly reliable assignment of bands of isoforms of erythropoietin separated by capillary electrophoresis

被引：27

作者：

Lacunza, I ^{[1
]}

Lara-Quintanar, P ^{[1
]}

Moya, G ^{[1
]}

Sanz, J ^{[1
]}

Diez-Masa, JC ^{[1
]}

de Frutos, M ^{[1
]}

机构：

[1] CSIC, Inst Organ Chem, E-28006 Madrid, Spain

来源：

ELECTROPHORESIS | 2004年 / 25卷 / 10-11期

关键词：

capillary electrophoresis; erythropoietin; migration index;

D O I：

10.1002/elps.200305831

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary zone electrophoresis of samples of recombinant human erythropoietin is performed. An in-house computer program is developed to compare the reliability of different migration parameters to assign the close migration bands of isoforms of erythropoietin. The migration time relative to the electroosmotic flow marker and the effective electrophoretic mobility are selected as the most accurate parameters. Percentages of correct assignment of bands higher than 99% are obtained with these parameters even when changes in operational factors are introduced. The chosen parameters have been applied to assign bands of isoforms in commercial samples of alpha- and beta-epoetin. The same capillary electrophoresis method has been applied to separate bands of isoforms of an erythropoietin analogue, darbepoetin alpha, the novel erythropoiesis-stimulating protein.

引用

页码：1569 / 1579

页数：11

共 25 条

[1]

ABRAMOWITZ M, 1965, HDB MATH FUNCTIONS, P297

[2]

Boone CM, 2000, ELECTROPHORESIS, V21, P1545, DOI 10.1002/(SICI)1522-2683(20000501)21:8<1545::AID-ELPS1545>3.0.CO

[3]

2-6

[4] ELECTROPHORETIC SELECTIVITY AS A FUNCTION OF OPERATING PARAMETERS IN FREE SOLUTION CAPILLARY ELECTROPHORETIC SEPARATION OF DIPEPTIDES [J].