Analysis of DNA curvature distribution in mycobacterial promoters using theoretical models

被引：8

作者：

Kalate, RN

Kulkarni, BD ^{[1
]}

Nagaraja, V

机构：

[1] Natl Chem Lab, Div Chem Engn, Pune 411008, Maharashtra, India

[2] Indian Inst Sci, Microbiol & Cell Biol Dept, Bangalore 012, Karnataka, India

来源：

BIOPHYSICAL CHEMISTRY | 2002年 / 99卷 / 01期

关键词：

mycobacterial promoters; transcription; curvature models;

D O I：

10.1016/S0301-4622(02)00124-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, 125 different mycobacterial promoters are analyzed for their DNA curvature distribution using several di- and tri-nucleotide dependent models of DNA curvature. Different models give similar behavior and therefore qualitative validation of the results. Mycobacterial promoters resembling the E. coli sigma(70) type have almost 81% (85%) sequences having medium and high curvature profiles using dinucleotide-dependent models. Non-E. coli sigma(70) type mycobacterial promoters have comparatively higher percent of low curvature profiles. Very few extended -10 promoters have low curvature profiles. Mycobacterial promoters having A(n)T(m)(n + m greater than or equal to 3) tract in the upstream region of -35 box and repeated in phase with each other have high curvature profiles. M. smegmatis promoters have high curvature profiles compared to M. tuberculosis promoters. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：77 / 97

页数：21

共 40 条

[1] Upstream A-tracts increase bacterial promoter activity through interactions with the RNA polymerase α subunit [J].