Stage-specific expression of fuco-neolacto- (Lewis X) and ganglio-series neutral glycosphingolipids during brain development: Characterization of Lewis X and related glycosphingolipids in bovine, human and rat brain

被引：21

作者：

Dasgupta, S ^{[1
]}

Hogan, EL ^{[1
]}

Spicer, SS ^{[1
]}

机构：

[1] MED UNIV S CAROLINA, DEPT PATHOL & LAB MED, CHARLESTON, SC 29425 USA

来源：

GLYCOCONJUGATE JOURNAL | 1996年 / 13卷 / 03期

关键词：

neutral glycosphingolipid; brain development; stage-specific expression; gas chromatography mass spectrometry;

D O I：

10.1007/BF00731469

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have purified and characterized a bovine brain pentaglycosylceramide as Lewis X and identified it in human and rat brain using anti-Lewis X (anti-SSEA 1) monoclonal antibody. Neutral glycosphingolipid expression in developing rat brain has been examined by digoxigenin immunostaining and TLC-immunostaining using anti-SSEA 1 and anti-GgOse(4)Cer (GA1) monoclonal antibodies. Five transient Lewis X-series bands were identified in brain at embryonic day 15 that disappear by postnatal day 5 (one disappears at embryonic day 18). Gangliotetraosylceramide (GA1) first appears at embryonic day 21 and increases in concentration with age until postnatal day 21. In addition, we have purified another minor brain neutral glycosphingolipid and tentatively identified it as a Lewis X-series glycolipid by gas chromatography-mass spectrometry analysis followed by TLC-immunostaining; with anti-SSEA 1 antibody.

引用

页码：367 / 375

页数：9

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