Immobilization of a cyclodextrin glucanotransferase (CGTase) onto polyethylene film with a carboxylic acid group and production of cyclodextrins from corn starch using CGTase-immobilized PE film

被引：13

作者：

Choi, SH

Kim, MS

Ryoo, JJ

Lee, KP ^{[1
]}

Shin, HD

Kim, SH

Lee, YH

机构：

[1] Kyungpook Natl Univ, Grad Sch, Dept Chem, Taegu 702701, South Korea

[2] Coll Nat Sci, Dept Genet Engn, Taegu 702701, South Korea

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 2002年 / 85卷 / 11期

关键词：

immobilization; cyclodextrin glucanotransferase (CGTase); radiation-induced grafting copolymerization; polyethylene film; acrylic acid; cyclodextrins (CDs);

D O I：

10.1002/app.10879

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Carboxylic acid groups were introduced onto polyethylene (PE) film by radiation-induced graft copolymerization. Subsequently, the clodextrin glucanotransferase (CGTase) was immobilized on the PE film with a carboxylic acid group. The activity of the immobilized CGTase on PE film was in the range of 0.40-1.04 U/cm(2) per min. The production of cyclodextrins (CDs) from corn starch was examined using the CGTase-immobilized PE film. The production ratios of CDs using CGTase-immobilized PE film was in the following order: alpha-CD > beta-CD > gamma-CD. (C) 2002 Wiley Periodicals, Inc.

引用

页码：2451 / 2457

页数：7

共 22 条

[1] Addition of polar organic solvents can improve the product selectivity of cyclodextrin glycosyltransferase - Solvent effects on CGTase [J].