Sequencing single molecules of DNA

被引：138

作者：

Bayley, Hagan ^{[1
]}

机构：

[1] Univ Oxford, Dept Chem, Chem Res Lab, Oxford OX1 3TA, England

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2006年 / 10卷 / 06期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.cbpa.2006.10.040

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In 2004, the NIH set a remarkable challenge: the $1000 genome. Roughly speaking, success would provide, by 2015, the ability to sequence the complete genome of an individual human, quickly and at an accessible price. An intermediate goal of a $100 000 genome was set for 2010. While the cost of Sanger sequencing has dropped dramatically over the past two decades, it is unlikely that the $100 000 genome will be achieved by this means. New massively parallel technologies will push the cost of sequencing towards this mark, but it is doubtful whether these efforts will match the $1000 goal. The best bets for ultrarapid, low-cost sequencing are single-molecule approaches.

引用

页码：628 / 637

页数：10

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