Single-cell technologies for monitoring immune systems

被引：294

作者：

Chattopadhyay, Pratip K. ^{[1
]}

Gierahn, Todd M. ^{[2
]}

Roederer, Mario ^{[1
]}

Love, J. Christopher ^{[2
]}

机构：

[1] NIAID, ImmunoTechnol Sect, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA

[2] MIT, Koch Inst Integrat Canc Res, Cambridge, MA 02139 USA

来源：

NATURE IMMUNOLOGY | 2014年 / 15卷 / 02期

关键词：

NATURAL-KILLER-CELLS; FLOW-CYTOMETRY; MIGRATION BEHAVIOR; GENE-EXPRESSION; MASS CYTOMETRY; ACTIVATION; ANTIBODIES; SECRETION; HIERARCHY; RESPONSES;

D O I：

10.1038/ni.2796

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The complex heterogeneity of cells, and their interconnectedness with each other, are major challenges to identifying clinically relevant measurements that reflect the state and capability of the immune system. Highly multiplexed, single-cell technologies may be critical for identifying correlates of disease or immunological interventions as well as for elucidating the underlying mechanisms of immunity. Here we review limitations of bulk measurements and explore advances in single-cell technologies that overcome these problems by expanding the depth and breadth of functional and phenotypic analysis in space and time. The geometric increases in complexity of data make formidable hurdles for exploring, analyzing and presenting results. We summarize recent approaches to making such computations tractable and discuss challenges for integrating heterogeneous data obtained using these single-cell technologies.

引用

页码：128 / 135

页数：8

共 63 条

[1]

Aghaeepour N, 2013, NAT METHODS, V10, P228, DOI [10.1038/nmeth.2365, 10.1038/NMETH.2365]

[2] RchyOptimyx: Cellular hierarchy optimization for flow cytometry [J].

Aghaeepour, Nima ;

Jalali, Adrin ;

O'Neill, Kieran ;

Chattopadhyay, Pratip K. ;

Roederer, Mario ;

Hoos, Holger H. ;

Brinkman, Ryan R. .

CYTOMETRY PART A, 2012, 81A (12) :1022-1030

[3] Early immunologic correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assays* [J].

Aghaeepour, Nima ;

Chattopadhyay, Pratip K. ;

Ganesan, Anuradha ;

O'Neill, Kieran ;

Zare, Habil ;

Jalali, Adrin ;

Hoos, Holger H. ;

Roederer, Mario ;

Brinkman, Ryan R. .

BIOINFORMATICS, 2012, 28 (07) :1009-1016

[4] A multiplex assay for the simultaneous detection of antibodies against 15 Plasmodium falciparum and Anopheles gambiae saliva antigens [J].

Ambrosino, Elena ;

Dumoulin, Chloe ;

Orlandi-Pradines, Eve ;

Remoue, Franck ;

Toure-Balde, Aissatou ;

Tall, Adama ;

Sarr, Jean Biram ;

Poinsignon, Anne ;

Sokhna, Cheikh ;

Puget, Karine ;

Trape, Jean-Francois ;

Pascual, Aurelie ;

Druilhe, Pierre ;

Fusai, Thierry ;

Rogier, Christophe .

MALARIA JOURNAL, 2010, 9

[5] viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia [J].

Amir, El-ad David ;

Davis, Kara L. ;

Tadmor, Michelle D. ;

Simonds, Erin F. ;

Levine, Jacob H. ;

Bendall, Sean C. ;

Shenfeld, Daniel K. ;

Krishnaswamy, Smita ;

Nolan, Garry P. ;

Pe'er, Dana .

NATURE BIOTECHNOLOGY, 2013, 31 (06) :545-+

[6] Phenotype and Function of Human T Lymphocyte Subsets: Consensus and Issues [J].

Appay, Victor ;

van Lier, Rene A. W. ;

Sallusto, Federica ;

Roederer, Mario .

CYTOMETRY PART A, 2008, 73A (11) :975-983

[7] A deep profiler's guide to cytometry [J].

Bendall, Sean C. ;

Nolan, Garry P. ;

Roederer, Mario ;

Chattopadhyay, Pratip K. .

TRENDS IN IMMUNOLOGY, 2012, 33 (07) :323-332

[8] Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum [J].

Bendall, Sean C. ;

Simonds, Erin F. ;

Qiu, Peng ;

Amir, El-ad D. ;

Krutzik, Peter O. ;

Finck, Rachel ;

Bruggner, Robert V. ;

Melamed, Rachel ;

Trejo, Angelica ;

Ornatsky, Olga I. ;

Balderas, Robert S. ;

Plevritis, Sylvia K. ;

Sachs, Karen ;

Pe'er, Dana ;

Tanner, Scott D. ;

Nolan, Garry P. .

SCIENCE, 2011, 332 (6030) :687-696

[9] Quantitative analysis of protein co-localization on B cells opsonized with rituximab and complement using the ImageStream multispectral imaging flow cytometer [J].

Beum, Paul V. ;

Lindorfer, Margaret A. ;

Hall, Brian E. ;

George, Thaddeus C. ;

Frost, Keith ;

Morrissey, Philip J. ;

Taylor, Ronald P. .

JOURNAL OF IMMUNOLOGICAL METHODS, 2006, 317 (1-2) :90-99

[10] Multiplexed mass cytometry profiling of cellular states perturbed by small-molecule regulators [J].

Bodenmiller, Bernd ;

Zunder, Eli R. ;

Finck, Rachel ;

Chen, Tiffany J. ;

Savig, Erica S. ;

Bruggner, Robert V. ;

Simonds, Erin F. ;

Bendall, Sean C. ;

Sachs, Karen ;

Krutzik, Peter O. ;

Nolan, Garry P. .

NATURE BIOTECHNOLOGY, 2012, 30 (09) :858-U89

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