A novel type A2 neurotoxin gene cluster in Clostridium botulinum strain Mascarpone

被引：21

作者：

Franciosa, Giovanna ^{[1
]}

Maugliani, Antonella ^{[1
]}

Floridi, Francesca ^{[1
]}

Aureli, Paolo ^{[1
]}

机构：

[1] Natl Ctr Food Qual & Risk Assessment, Lab Food Mibrobial Hazards, Ist Super Sanita, I-00161 Rome, Italy

来源：

FEMS MICROBIOLOGY LETTERS | 2006年 / 261卷 / 01期

关键词：

Clostridium botulinum; boNT/A gene cluster; nucleotide sequence;

D O I：

10.1111/j.1574-6968.2006.00331.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The partial nucleotide sequence (similar to 10 kb) of the cluster of genes encoding the botulinum neurotoxin complex in Clostridium botulinum type A strain Mascarpone was determined. The analysis revealed six ORFs (orfs), which were organized as in the type A2 and type A3 botulinum neurotoxin gene clusters of strains Kyoto-F and NCTC 2916, respectively. While the orfs at the proximal and distal ends of the sequence (orfX2 and bont/A genes) shared a high level of similarity with the corresponding sequences of strain Kyoto-F, the segment encompassing the orfX1 and botR/A genes within the sequence exhibited a higher degree of homology to the related region in strain NCTC 2916. The mosaic structure of the Mascarpone neurotoxin gene cluster suggests recombinational exchanges.

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页码：88 / 94

页数：7

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