Connexin43 mRNA contains a functional internal ribosome entry site

被引：71

作者：

Schiavi, A ^{[1
]}

Hudder, A ^{[1
]}

Werner, R ^{[1
]}

机构：

[1] Univ Miami, Sch Med, Dept Biochem & Mol Biol, Miami, FL 33101 USA

来源：

FEBS LETTERS | 1999年 / 464卷 / 03期

关键词：

connexin43; mRNA; translation control; internal ribosome entry site;

D O I：

10.1016/S0014-5793(99)01699-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A reporter gene construct,vas used to study the regulation of connexin43 (Cx43) expression, the major gap junction protein found in heart and uterus, in transfected cell lines. The construct had the firefly luciferase gene under the control of the Cx43 promoter. Inclusion of the 5'-untranslated region (UTR) of the mRNA in the construct increased luciferase expression by 70%, A bicistronic vector assay demonstrated that the Cx43 5'-UTR contains a strong internal ribosome entry site (IRES), Deletion analysis localized the IRES element to the upstream portion of the 5'-UTR, (C) 1999 Federation of European Biochemical Societies.

引用

页码：118 / 122

页数：5

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