Connexin43 mRNA contains a functional internal ribosome entry site

被引：71

作者：

Schiavi, A ^{[1
]}

Hudder, A ^{[1
]}

Werner, R ^{[1
]}

机构：

[1] Univ Miami, Sch Med, Dept Biochem & Mol Biol, Miami, FL 33101 USA

来源：

FEBS LETTERS | 1999年 / 464卷 / 03期

关键词：

connexin43; mRNA; translation control; internal ribosome entry site;

D O I：

10.1016/S0014-5793(99)01699-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A reporter gene construct,vas used to study the regulation of connexin43 (Cx43) expression, the major gap junction protein found in heart and uterus, in transfected cell lines. The construct had the firefly luciferase gene under the control of the Cx43 promoter. Inclusion of the 5'-untranslated region (UTR) of the mRNA in the construct increased luciferase expression by 70%, A bicistronic vector assay demonstrated that the Cx43 5'-UTR contains a strong internal ribosome entry site (IRES), Deletion analysis localized the IRES element to the upstream portion of the 5'-UTR, (C) 1999 Federation of European Biochemical Societies.

引用

页码：118 / 122

页数：5

共 24 条

[11] Immunoglobulin binding protein (BiP) function is required to protect cells from endoplasmic reticulum stress but is not required for the secretion of selective proteins [J].

Morris, JA ;

Dorner, AJ ;

Edwards, CA ;

Hendershot, LM ;

Kaufman, RJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (07) :4327-4334

[12] Translation initiation of a cardiac voltage-gated potassium channel by internal ribosome entry [J].

Negulescu, D ;

Leong, LEC ;

Chandy, KG ;

Semler, BL ;

Gutman, GA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (32) :20109-20113

[13] Expression of connexin-43 and connexin-26 in the rat myometrium during pregnancy and labor is differentially regulated by mechanical and hormonal signals [J].

Ou, CW ;

Orsino, A ;

Lye, SJ .

ENDOCRINOLOGY, 1997, 138 (12) :5398-5407

[14] CIS-ACTING TRANSLATIONAL EFFECTS OF THE 5' NONCODING REGION OF C-MYC MESSENGER-RNA [J].

PARKIN, N ;

DARVEAU, A ;

NICHOLSON, R ;

SONENBERG, N .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (07) :2875-2883

[15] INTERNAL INITIATION OF TRANSLATION OF EUKARYOTIC MESSENGER-RNA DIRECTED BY A SEQUENCE DERIVED FROM POLIOVIRUS RNA [J].

PELLETIER, J ;

SONENBERG, N .

NATURE, 1988, 334 (6180) :320-325

[16] INCREASE IN MESSENGER-RIBONUCLEIC-ACID ENCODING THE MYOMETRIAL GAP JUNCTION PROTEIN, CONNEXIN-43, REQUIRES PROTEIN-SYNTHESIS AND IS ASSOCIATED WITH INCREASED EXPRESSION OF THE ACTIVATOR PROTEIN-1, C-FOS [J].

PIERSANTI, M ;

LYE, SJ .

ENDOCRINOLOGY, 1995, 136 (08) :3571-3578

[17] UNIQUE FEATURES OF INTERNAL INITIATION OF HEPATITIS-C VIRUS-RNA TRANSLATION [J].

REYNOLDS, JE ;

KAMINSKI, A ;

KETTINEN, HJ ;

GRACE, K ;

CLARKE, BE ;

CARROLL, AR ;

ROWLANDS, DJ ;

JACKSON, RJ .

EMBO JOURNAL, 1995, 14 (23) :6010-6020

[18]

SMITH JH, 1991, AM J PATHOL, V139, P801

[19] Translation of vascular endothelial growth factor mRNA by internal ribosome entry: Implications for translation under hypoxia [J].

Stein, I ;

Itin, A ;

Einat, P ;

Skaliter, R ;

Grossman, Z ;

Keshet, E .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (06) :3112-3119

[20] C-Myc 5′ untranslated region contains an internal ribosome entry segment [J].

Stoneley, M ;

Paulin, FEM ;

Le Quesne, JPC ;

Chappell, SA ;

Willis, AE .

ONCOGENE, 1998, 16 (03) :423-428

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