Genetic tags for labelling live cells: gap junctions and beyond

被引：12

作者：

Falk, MM ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

TRENDS IN CELL BIOLOGY | 2002年 / 12卷 / 09期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0962-8924(02)02343-7

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The availability of green fluorescent protein (GFP) as a tracer for observing proteins in living cells has revolutionized cell biology and spurred an intensive search for GFP variants with novel characteristics, additional autofluorescent proteins and alternative techniques of protein labelling. Two recent studies-one on tagging with tetracysteine motifs and labelling with biarsenic fluorophores of different colours, and the other on GFP tagging and fluorescence recovery after photobleaching (FRAP) - show how membrane channels are added and removed from gap junctions by using different fluorescent tags to distinguish between newly synthesized and older protein populations.

引用

页码：399 / 404

页数：6

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