Autocrine catecholamine biosynthesis and the β2-adrenoceptor signal promote pigmentation in human epidermal melanocytes

被引:95
作者
Gillbro, JM
Marles, LK
Hibberts, NA
Schallreuter, KU [1 ]
机构
[1] Univ Bradford, Dept Biomed Sci, Bradford BD7 1DP, W Yorkshire, England
[2] Ernst Moritz Arndt Univ Greifswald, Inst Pigmentary Disorders, Greifswald, Germany
[3] Univ Bradford, Inst Pigmentary Disorders, Bradford BD7 1DP, W Yorkshire, England
关键词
beta(2)-adrenoceptors; cAMP; catecholamine biosynthesis; melanocytes;
D O I
10.1111/j.0022-202X.2004.23210.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Earlier it has been shown that human proliferating/undifferentiated basal keratinocytes hold the full capacity for autocrine catecholamine synthesis/degradation and express beta(2)-adrenoceptors (beta(2)-AR). In this report, we show that human melanocytes also express all of the mRNA and enzymes for autocrine synthesis of norepinephrine but fail to produce epinephrine. So far, it was established that human melanocytes express alpha(1)-AR which are induced by norepinephrine yielding the inosine triphosphate diacylglycerol signal. The presence of catecholamine synthesis and the beta(2)-AR signal escaped definition at that time. Using RT-PCR, immunofluorescence and radioligand binding with the beta(2)-AR antagonist (-)-[H-3]CGP 12177, we show here that human melanocytes express functional beta(2)-AR (4230 receptors per cell) with a B-max at 129.3 and a K-D of 3.19 nM but lack beta(1)-AR expression. beta(2)-AR stimulation with epinephrine 10(-6) M and salbutamol 10(-6)-10(-5) M yielded a strong cyclic adenosine monophospate (cAMP) response in association with upregulated melanin production. Taken together these results indicate that the biosynthesis and release of epinephrine (10(-6) M) by surrounding keratinocytes can provide the cAMP response leading to melanogenesis in melanocytes via the beta(2)-AR signal. Moreover, the discovery of this catecholaminergic cAMP response in melanocytes adds a new source for this important second messenger in melanogenesis.
引用
收藏
页码:346 / 353
页数:8
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