A DNA probe specific for Aeromonas colonies

被引：60

作者：

Chacón, MR

Castro-Escarpulli, G

Soler, L

Guarro, J

Figueras, MJ

机构：

[1] Univ Rovira & Virgili, Fac Med & Ciencies Salud, Dept Ciencias Medicas Basicas, Reus 43201, Spain

[2] Escuela Natl Ciencias Biol, Dept Bacteriol, Mexico City, DF, Mexico

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2002年 / 44卷 / 03期

关键词：

D O I：

10.1016/S0732-8893(02)00455-8

中图分类号：

R51 [传染病];

学科分类号：

100401 [流行病与卫生统计学];

摘要：

Members of the genus Aeromonas are important enteropathogens. Commercial identification systems are often unable to correctly identify Aeromonas strains and misidentification as Vibrio spp. is common. A digoxigenin-DNA probe based on a 237 bp of the glycerophospholipid-cholesterol acyltransferase gene has been tested in a colony hybridization assay. The probe hybridized with all Aeromonas species tested (n = 16) but not with strains of other enteropathogenic bacteria (n = 20). The probe allowed the unequivocal identification of Aeromonas in primary isolation media within 36 h. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：221 / 225

页数：5

共 22 条

[1]

Misidentification of unusual Aeromonas species as members of the genus Vibrio:: A continuing problem [J].