Sequence analysis of the vir-region from Agrobacterium tumefaciens octopine Ti plasmid pTi15955

被引：16

作者：

Schrammeijer, B

Beijersbergen, A

Idler, KB

Melchers, LS

Thompson, DV

Hooykaas, PJJ

机构：

[1] Leiden Univ, Clusius Lab, Inst Mol Plant Sci, NL-2333 AL Leiden, Netherlands

[2] Agrigenet Corp, Adv Res Div, Madison, WI 53716 USA

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2000年 / 51卷 / 347期

关键词：

Agrobacterium tumefaciens; sequence; virulence genes;

D O I：

10.1093/jexbot/51.347.1167

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The nucleotide sequence of 42 775 bp of the vir-region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955 is reported here. Although the nucleotide sequences of several parts of this region from this or closely related plasmids have been published previously, the present work establishes for the first time the complete arrangement of all the essential Virulence genes and their intergenic regions of an octopine Ti plasmid, The disruption of some of the intergenic areas by insertion (IS) elements is typical for the octopine Ti plasmids. Several new ORFs were identified, including ORFs immediately downstream of virD4 and virE2, which probably represent new genes involved in virulence.

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页码：1167 / 1169

页数：3

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