Comparison between antigen-capture ELISA and conventional methods used for titration of infectious bursal disease virus

被引：7

作者：

Hassan, MK

Saif, YM

Shawky, S

机构：

[1] OHIO STATE UNIV,OHIO AGR RES & DEV CTR,FOOD ANIM HLTH RES PROGRAM,WOOSTER,OH 44691

[2] CORNELL DUCK RES LAB,EASTPORT,NY 11941

来源：

AVIAN DISEASES | 1996年 / 40卷 / 03期

关键词：

infectious bursal disease virus; titration; ELISA;

D O I：

10.2307/1592264

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Two antigen-capture enzyme-linked immunosorbent assays (polyclonal and monoclonal AC-ELISAs) were developed and evaluated for titration of infectious bursal disease viruses (IBDVs) propagated in different host systems, namely BGM-70 continuous cell line, primary chicken embryo fibroblast cells, and chicken bursa of Fabricius. The polyclonal system was more sensitive (P < 0.05) than the monoclonal system but both were specific as indicated by the negative results obtained with three non-IBDVs. The results revealed chat the conventional systems used for titration of IBDVs (cell cultures and embryonating chicken eggs) were more sensitive than the polyclonal AC-ELISA.

引用

页码：562 / 566

页数：5

共 19 条

[1]

BUTLER JE, 1987, FASEB J, V46, P2548

[2]

CHEVILLE NF, 1967, AM J PATHOL, V51, P527

[3]

HASSAN MK, 1996, AVIAN DIS, V40, P147

[4] INFECTIVITY OF INFECTIOUS BURSAL DISEASE VIRUS FOR EMBRYONATING EGGS [J].