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Comparison between antigen-capture ELISA and conventional methods used for titration of infectious bursal disease virus

被引：7
作者：
Hassan, MK
Saif, YM
Shawky, S
机构：
[1] OHIO STATE UNIV,OHIO AGR RES & DEV CTR,FOOD ANIM HLTH RES PROGRAM,WOOSTER,OH 44691
[2] CORNELL DUCK RES LAB,EASTPORT,NY 11941
来源：
AVIAN DISEASES | 1996年 / 40卷 / 03期
关键词：
infectious bursal disease virus; titration; ELISA;
D O I：
10.2307/1592264
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Two antigen-capture enzyme-linked immunosorbent assays (polyclonal and monoclonal AC-ELISAs) were developed and evaluated for titration of infectious bursal disease viruses (IBDVs) propagated in different host systems, namely BGM-70 continuous cell line, primary chicken embryo fibroblast cells, and chicken bursa of Fabricius. The polyclonal system was more sensitive (P < 0.05) than the monoclonal system but both were specific as indicated by the negative results obtained with three non-IBDVs. The results revealed chat the conventional systems used for titration of IBDVs (cell cultures and embryonating chicken eggs) were more sensitive than the polyclonal AC-ELISA.
引用
收藏
页码：562 / 566
页数：5
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