ELISA-quantitation of phosphorylated tau protein in the Alzheimer's disease brain

被引：27

作者：

Herrmann, M

Golombowski, S

Kräuchi, K

Frey, P

Mourton-Gilles, C

Hulette, C

Rosenberg, C

Müller-Spahn, F

Hock, C

机构：

[1] Univ Basel, Dept Psychiat, Basel, Switzerland

[2] Novartis, Basel, Switzerland

[3] Univ Montpellier, Dept Pharm, CNRS, F-34059 Montpellier, France

[4] Duke Univ, Ctr Med, Kathleen Price Bryan Brain Bank, Durham, NC USA

来源：

EUROPEAN NEUROLOGY | 1999年 / 42卷 / 04期

关键词：

dementia; biochemical markers; early diagnosis; neurofibrillary tangles;

D O I：

10.1159/000008108

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

A reliable, sensitive and specific sandwich ELISA for the quantitation of paired helical filament (PHF) tau in human brain was developed using well-defined monoclonal antibodies. We examined rapid-autopsy-derived brain tissue from 21 neuropathologically confirmed Alzheimer's disease (AD) patients and 14 nondemented controls, matched for age, sex and postmortem delay times, We demonstrated significant elevations of phosphorylated tau levels in the frontal and parietal cortex as well as in the hippocampus of AD patients as compared to the nondemented controls. No difference was observed in the cerebellum. Phosphorylated tau levels measured by ELISA were significantly correlated with the presence or absence of neurofibrillary tangles. Copyright (C) 1999 S. Karger AG, Basel.

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页码：205 / 210

页数：6

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