Assembly and structural characterization of an authentic complex between human follicle stimulating hormone and a hormone-binding ectodomain of its receptor

Follicle stimulating hormone (FSH) is secreted from the pituitary gland to regulate reproduction in vertebrates. FSH signals through a G-protein coupled receptor (FSHR) on the target cell Surface. We describe here the strategy to produce a soluble FSH-FSHR complex that involves the C co-secretion of a truncated FSHR ectodomain (FSHRHB) and a covalently linked FSH alpha beta heterodimer from baculovirus-infected insect cells. FSH binds to FSHRHB with a high affinity comparable to that for the full-length receptor. The crystal structure of the FSH-FSHRHB complex provides explanations for the high affinity and specificity of FSH interaction with FSHR, and it shows an unexpected dimerization of these complexes. Here we also compare the crystal structure with theoretical models of the FSH-FSHR-binding mode. We conclude that the FSH-FSHRHB structure gives an authentic representation of FSH binding to intact FSHR. (c) 2006 Elsevier Ireland Ltd. All rights reserved.