Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library

被引：97

作者：

Mena, Marco A.

Treynor, Thomas P.

Mayo, Stephen L.

Daugherty, Patrick S. ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Chem Engn, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Inst Collaborat Biotechnol, Santa Barbara, CA 93106 USA

[3] CALTECH, Div Biol, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

NATURE BIOTECHNOLOGY | 2006年 / 24卷 / 12期

关键词：

D O I：

10.1038/nbt1264

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The utility of blue fluorescent protein ( BFP) has been limited by its low quantum yield and rapid photobleaching. A library targeting residues neighboring the chromophore yielded a variant with enhanced quantum yield ( 0.55 versus 0.34), reduced pH sensitivity and a 40-fold increase in photobleaching half-life. This BFP, named Azurite, is well expressed in bacterial and mammalian cells and extends the palette of fluorescent proteins that can be used for imaging.

引用

页码：1569 / 1571

页数：3

共 20 条

[1]

Bessette Paul H, 2003, Methods Mol Biol, V231, P29

[2] A monomeric red fluorescent protein [J].