DNA Methylation in Embryonic Stem Cells

被引：63

作者：

Altun, Gulsah ^{[1
,2
]}

Loring, Jeanne F. ^{[2
]}

Laurent, Louise C. ^{[1
,2
]}

机构：

[1] Univ Calif San Diego, Dept Reprod Med, San Diego, CA 92103 USA

[2] Scripps Res Inst, Ctr Regenerat Med, Dept Physiol Chem, La Jolla, CA USA

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2010年 / 109卷 / 01期

关键词：

DNA METHYLATION; EMBRYONIC STEM CELLS; EPIGENETICS; MICROARRAY; NEXT-GENERATION SEQUENCING; CPG ISLANDS; WIDE; DNMT3A; GENE; PLURIPOTENT; MAINTENANCE; EPIGENETICS; SITES; ASSAY; MAPS;

D O I：

10.1002/jcb.22374

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Embryonic stem cells (ESCs) are pluripotent, self-renewing cells. These cells can be used in applications such as cell therapy, drug development, disease modeling, and the study of cellular differentiation. Investigating the interplay of epigenetics, genetics, and gene expression in control Of pluripotence and differentiation Could give important insights on how these cells function. One of the best known epigenetic Factors is DNA methylation, which is a major mechanism for regulation of gene expression. This phenomenon is mostly seen in imprinted genes and X-chromosome inactivation where DNA methylation of promoter regions leads to repression of gene expression. Differential DNA methylation of pluripotence-associated genes Such as Nanog and 0ct4/Pou5f1 has been observed between pluripotent and differentiated cells. It is clear that tight regulation of DNA methylation is necessary for normal development. As more associations between aberrant DNA methylation and disease are reported, the demand for high-throughput approaches for DNA methylation analysis has increased. In this article, we highlight these methods and discuss recent DNA methylation studies on ESCs. J. Cell. Biochem. 109: 1-6, 2010. (C) 2009 Wiley-Liss, Inc.

引用

页码：1 / 6

页数：6

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