Receptors for bradykinin and prostaglandin E-2 coupled to Ca2+ signalling in rat cortical collecting duct

被引:20
作者
AnkorinaStark, I [1 ]
Haxelmans, S [1 ]
Schlatter, E [1 ]
机构
[1] UNIV MUNSTER,MED POLIKLIN,D-48149 MUNSTER,GERMANY
关键词
D O I
10.1016/S0143-4160(97)90065-8
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In freshly isolated rat cortical collecting ducts (CCD) we measured intracellular Ca2+ activity ([Ca2+](i)) with the Fura-2 method, Bradykinin (BK) induced a transient and biphasic increase in [Ca2+](i). This increase was concentration dependent and was half maximal at a concentration of 15 nM. The B-2 receptor antagonist HOE 140 (100 nM, n = 6) completely abolished BK (100 nM) induced increase in [Ca2+](i). The B-1 receptor agonist des-Arg(9)-bradykinin (100 nM, n = 4) had no effect on [Ca2+](i). In the absence of extracellular Ca2+, the maximal increase in [Ca2+](i) induced by BK was diminished and the secondary plateau phase was completely abolished. Prostaglandin E-2 (PGE(2)) elevated [Ca2+](i) also concentration-dependently and biphasically. A half maximal effect was reached with 1 nM PGE(2). The secondary plateau phase was absent when extracellular Ca2+ was removed. Sulprostone (100 nM, n = 6) mimicked the PGE(2) (100 nM) induced increase in [Ca2+](i). The effect of BK (100 nM) on [Ca2+](i) was not inhibited by the cyclooxygenase inhibitor indomethacin (10 mu M, n = 5). Dopamine (1 mu M, n = 4) did not significantly alter [Ca2+](i). BK and PGE(2) regulate [Ca2+](i) in the rat CCD via release of Ca2+ from intracellular Ca2+ stores as well as via Ca2+ influx from extracellular space. BK directly modulates [Ca2+], through B-2 receptors. EP1 receptors are most likely to be responsible for the PGE(2) induced increase in [Ca2+](i).
引用
收藏
页码:269 / 275
页数:7
相关论文
共 41 条
[21]   PROSTAGLANDIN-E2 PRODUCTION IN RAT IMCD CELLS .1. STIMULATION BY DOPAMINE [J].
HUO, TH ;
HEALY, DP .
AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (04) :F647-F654
[22]  
MCGIFF JC, 1976, FED PROC, V35, P175
[23]   PHYSIOLOGY OF THE MESANGIAL CELL [J].
MENE, P ;
SIMONSON, MS ;
DUNN, MJ .
PHYSIOLOGICAL REVIEWS, 1989, 69 (04) :1347-1424
[24]   Bradykinin B-2 receptor antagonist increases chloride and water absorption in rat medullary collecting duct [J].
Mukai, H ;
Fitzgibbon, WR ;
Bozeman, G ;
Margolius, HS ;
Ploth, DW .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 1996, 271 (02) :R352-R360
[25]   ANTIDIURETIC-HORMONE ACTS VIA V1 RECEPTORS ON INTRACELLULAR CALCIUM IN THE ISOLATED PERFUSED RABBIT CORTICAL THICK ASCENDING LIMB [J].
NITSCHKE, R ;
FROBE, U ;
GREGER, R .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1991, 417 (06) :622-632
[26]  
ROUCH AJ, 1991, AM J PHYSIOL, V260, pF86
[27]  
ROUCH AL, 1993, AM J PHYSIOL, V263, pF569
[28]   LUMINAL PROSTAGLANDIN-E RECEPTORS REGULATE SALT AND WATER TRANSPORT IN RABBIT CORTICAL COLLECTING DUCT [J].
SAKAIRI, Y ;
JACOBSON, HR ;
NOLAND, TD ;
BREYER, MD .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY, 1995, 269 (02) :F257-F265
[29]   ION CONDUCTANCES OF ISOLATED CORTICAL COLLECTING DUCT CELLS [J].
SCHLATTER, E ;
FROBE, U ;
GREGER, R .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1992, 421 (04) :381-387
[30]  
SCHLATTER E, 1995, J AM SOC NEPHROL, V6, P1223