Inheritance, gene expression, and lignin characterization in a mutant pine deficient in cinnamyl alcohol dehydrogenase

被引:171
作者
MacKay, JJ
OMalley, DM
Presnell, T
Booker, FL
Campbell, MM
Whetten, RW
Sederoff, RR
机构
[1] N CAROLINA STATE UNIV, DEPT FORESTRY, FOREST BIOTECHNOL GRP, RALEIGH, NC 27695 USA
[2] N CAROLINA STATE UNIV, DEPT GENET, RALEIGH, NC 27695 USA
[3] N CAROLINA STATE UNIV, DEPT WOOD & PAPER SCI, RALEIGH, NC 27695 USA
[4] N CAROLINA STATE UNIV, DEPT CROP SCI, RALEIGH, NC 27695 USA
关键词
D O I
10.1073/pnas.94.15.8255
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have discovered a mutant loblolly pine (Pinus taeda L.) in which expression of the gene encoding cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.195) is severely reduced, The products of CAD, cinnamyl alcohols, are the precursors of lignin, a major cell wall polymer of plant vascular tissues, Lignin composition in this mutant shows dramatic modifications, including increased incorporation of the substrate of CAD (coniferaldehyde), indicating that CAD may modulate lignin composition in pine. The recessive cad-n1 allele, which causes this phenotype, was discovered in a tree heterozygous for this mutant allele, It is inherited as a simple Mendelian locus that maps to the same genomic region as the end locus, In mutant plants, CAD activity and abundance of end RNA transcript are low, and free CAD substrate accumulates to a high level, The wood of the mutant is brown, whereas the wood in wild types is nearly white, The wood phenotype resembles that of brown midrib (bm) mutants and some transgenic plants in which xylem is red-brown due to a reduction in CAD activity, However, unlike transgenics with reduced CAD, the pine mutant has decreased lignin content, Wood in which the composition of lignin varies beyond previous expectations still provides vascular function and mechanical support.
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页码:8255 / 8260
页数:6
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