A p53-dependent mechanism underlies macrocytic anemia in a mouse model of human 5q-syndrome

被引：269

作者：

Barlow, Jillian L. ^{[1
]}

Drynan, Lesley F. ^{[1
]}

Hewett, Duncan R. ^{[1
]}

Holmes, Luke R. ^{[1
]}

Lorenzo-Abalde, Silvia ^{[1
]}

Lane, Alison L. ^{[1
]}

Jolin, Helen E. ^{[1
]}

Pannell, Richard ^{[1
]}

Middleton, Angela J. ^{[1
]}

Wong, See Heng ^{[1
]}

Warren, Alan J. ^{[1
,3
]}

Wainscoat, James S. ^{[2
]}

Boultwood, Jacqueline ^{[2
]}

McKenzie, Andrew N. J. ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[2] John Radcliffe Hosp, Nuffield Dept Clin Lab Sci, Leukaemia Res Fund Mol Haematol Unit, Oxford OX3 9DU, England

[3] Univ Cambridge, Dept Haematol, Cambridge, England

来源：

NATURE MEDICINE | 2010年 / 16卷 / 01期

关键词：

COMMONLY DELETED REGION; RIBOSOMAL-PROTEIN S19; TCOF1; GENE-PRODUCT; P53; RNA; ACTIVATION; COLLINSSYNDROME; TREACHER; HAPLOINSUFFICIENCY; IDENTIFICATION; INACTIVATION;

D O I：

10.1038/nm.2063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The identification of the genes associated with chromosomal translocation breakpoints has fundamentally changed understanding of the molecular basis of hematological malignancies. By contrast, the study of chromosomal deletions has been hampered by the large number of genes deleted and the complexity of their analysis. We report the generation of a mouse model for human 5q-syndrome using large-scale chromosomal engineering. Haploinsufficiency of the Cd74-Nid67 interval (containing Rps14, encoding the ribosomal protein S14) caused macrocytic anemia, prominent erythroid dysplasia and monolobulated megakaryocytes in the bone marrow. These effects were associated with defective bone marrow progenitor development, the appearance of bone marrow cells expressing high amounts of the tumor suppressor p53 and increased bone marrow cell apoptosis. Notably, intercrossing with p53-deficient mice completely rescued the progenitor cell defect, restoring common myeloid progenitor and megakaryocytic-erythroid progenitor, granulocyte-monocyte progenitor and hematopoietic stem cell bone marrow populations. This mouse model suggests that a p53-dependent mechanism underlies the pathophysiology of the 5q-syndrome.

引用

页码：59 / U93

页数：9

共 49 条

[1] CHD5 is a tumor suppressor at human 1p36 [J].