7SK RNA, a non-coding RNA regulating P-TEFb, a general transcription factor

被引:99
作者
Diribarne, Gaelle [1 ]
Bensaude, Olivier [1 ]
机构
[1] ENS, CNRS, UMR Regulat Express Genet 8541, F-75230 Paris 05, France
关键词
transcription; 7SK RNA; P-TEFb; HEXIM; LARP7; MePCE; cyclin T1; CDK9; HUMAN-IMMUNODEFICIENCY-VIRUS; POLYMERASE-II TRANSCRIPTION; ELONGATION-FACTOR-B; METHYLPHOSPHATE CAP STRUCTURE; T-BINDING DOMAIN; POSITIVE TRANSCRIPTION; CARDIAC-HYPERTROPHY; HUMAN-CELLS; K RNA; SECONDARY STRUCTURE;
D O I
10.4161/rna.6.2.8115
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human 7SK RNA is an abundant 331 nt nuclear transcript generated by RNA polymerase III. Binding of 7SK RNA to HEXIM 1/2 turns these proteins into inhibitors of P-TEFb (Positive Transcriptional Elongation Factor b). P-TEFb is required for RNA polymerase II transcription elongation. 7SK RNA is released from P-TEFb/HEXIM/7SK complexes upon an arrest in transcription and physiological stimulations such as cardiac hypertrophy, leading to P-TEFb activation. The released 7SK RNA associates a subset of heterogeneous nuclear ribonucleoproteins (hnRNP). 7SK RNA has been evolutionary conserved in vertebrates and homologues are found in annelid, mollusc and insect genomes. 7SK RNA folds into several hairpins that serve as specific platforms for binding proteins. It is stabilized by mono-methylation of its 5'-triphosphate group and binding of a specific La-Related protein, IARP7 at its 3' end. As the likely best characterized example, 7SK RNA is a paradigm for non-coding RNAs regulating transcription.
引用
收藏
页码:122 / 128
页数:7
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