Confirmation of the arginine-finger hypothesis for the GAP-stimulated GTP-hydrolysis reaction of Ras

被引：301

作者：

Ahmadian, MR ^{[1
]}

Stege, P ^{[1
]}

Scheffzek, K ^{[1
]}

Wittinghofer, A ^{[1
]}

机构：

[1] MAX PLANCK INST MOL PHYSIOL,D-44139 DORTMUND,GERMANY

来源：

NATURE STRUCTURAL BIOLOGY | 1997年 / 4卷 / 09期

关键词：

D O I：

10.1038/nsb0997-686

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RasGAPs supply a catalytic residue, termed the arginine finger, into the active site of Ras thereby stabilizing the transition state of the GTPase reaction and increasing the reaction rate by more than one thousand-fold, in good agreement with the structure of the Ras.RasGAP complex.

引用

页码：686 / 689

页数：4

共 44 条

[41] A UNIVERSAL EXPRESSION-PURIFICATION SYSTEM BASED ON THE COILED-COIL INTERACTION OF MYOSIN HEAVY-CHAIN
WOLBER, V
MAEDA, K
SCHUMANN, R
BRANDMEIER, B
WIESMULLER, L
WITTINGHOFER, A
[J]. BIO-TECHNOLOGY, 1992, 10 (08): : 900 - 904
[42] XU G, 1991, CELL, V63, P835
[43] THE NEUROFIBROMATOSIS TYPE-1 GENE ENCODES A PROTEIN RELATED TO GAP
XU, GF
OCONNELL, P
VISKOCHIL, D
CAWTHON, R
ROBERTSON, M
CULVER, M
DUNN, D
STEVENS, J
GESTELAND, R
WHITE, R
WEISS, R
[J]. CELL, 1990, 62 (03) : 599 - 608
[44] AlF3 mimics the transition state of protein phosphorylation in the crystal structure of nucleoside diphosphate kinase and MgADP
Xu, YW
Morera, S
Janin, J
Cherfils, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (08) : 3579 - 3583

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