Fabrication of DNA Microarrays with Poly(L-glutamic acid) Monolayers on Gold Substrates for SPR Imaging Measurements

被引：48

作者：

Chen, Yulin ^{[1
]}

Nguyen, Anh ^{[1
]}

Niu, Lifang ^{[1
]}

Corn, Robert M. ^{[1
]}

机构：

[1] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA

来源：

LANGMUIR | 2009年 / 25卷 / 09期

基金：

美国国家科学基金会;

关键词：

PLASMON RESONANCE MEASUREMENTS; HYBRIDIZATION ADSORPTION; MULTIPLEXED DETECTION; ESCHERICHIA-COLI; SURFACES; PROTEIN; ARRAYS; SPECTROSCOPY; FILMS; BINDING;

D O I：

10.1021/la804021t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Robust single-stranded DNA (ssDNA) microarrays are created by attaching amine-modified oligonucleotides to a monolayer of poly(L-glutamic acid) (pGlu) that is electrostatically adsorbed onto a chemically modified gold thin film. This surface attachment chemistry methodology is first characterized with a combination of polarization-modulation Fourier transform infrared (PM-FTIR) spectroscopy and surface plasmon resonance (SPR) angle shift measurements. SPR imaging (SPRI) measurements of these ssDNA microarrays are then used to study two surface bioaffinity interactions: (i) the quantitative hybridization adsorption of complementary ssDNA onto mixed ssDNA microarray elements and (ii) the adsorption of single-stranded binding protein (SSB) onto fully and partially hybridized DNA microarray elements. The Langmuir adsorption coefficient (K-Ads) Of SSB binding to ssDNA was determined to be (5.5 +/- 0.4) x 10(9) M-1.

引用

页码：5054 / 5060

页数：7

共 34 条

[1]

Adamsom A.W., 1997, Physical chemistry of surfaces

[2] A multistep chemical modification procedure to create DNA arrays on gold surfaces for the study of protein-DNA interactions with surface plasmon resonance imaging [J].