Direct detection of genomic DNA by enzymatically amplified SPR imaging measurements of RNA microarrays

被引：195

作者：

Goodrich, TT ^{[1
]}

Lee, HJ ^{[1
]}

Corn, RM ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 13期

关键词：

D O I：

10.1021/ja039823p

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel surface enzymatic reaction scheme that amplifies the optical response of RNA microarrays to the binding of complementary DNA is developed for the direct detection and analysis of genomic DNA. The enzyme RNase H is shown to selectively and repeatedly destroy RNA from DNA?RNA heteroduplexes on gold surfaces; when used in conjunction with the label-free technique of surface plasmon resonance (SPR) imaging, DNA oligonucleotides can be detected at a concentration of 1 fM. This enzymatically amplified SPR imaging methodology is then utilized to detect and identify the presence of the TSPY gene in human genomic DNA without PCR amplification. Copyright © 2004 American Chemical Society.

引用

页码：4086 / 4087

页数：2

共 9 条

[1] A multistep chemical modification procedure to create DNA arrays on gold surfaces for the study of protein-DNA interactions with surface plasmon resonance imaging [J].