Aphid transmission alters the genomic and defective RNA populations of Citrus tristeza virus isolates

被引:40
作者
Albiach-Martí, MR
Guerri, J
de Mendoza, AH
Laigret, F
Ballester-Olmos, JF
Moreno, P
机构
[1] Inst Valenciano Invest Agr, E-46113 Moncada, Valencia, Spain
[2] INRA, Lab Cellulaire & Mol, F-33883 Villenave Dornon, France
关键词
graft-inoculation;
D O I
10.1094/PHYTO.2000.90.2.134
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A total of 14 Spanish isolates of Citrus tristeza virus (CTV) and 1 isolate from Japan were transmitted by Aphis gossypii, and the subisolates obtained were compared with the source isolates for symptom expression and double-stranded RNA (dsRNA) pattern. Of the 14 Spanish isolates, 9 showed altered dsRNA patterns after aphid transmission but only minor variations in the intensity of symptoms induced on Mexican lime. Northern blot hybridization with complementary DNA (cDNA) probes corresponding to both the 5' and the 3' termini of the CTV genomic RNA (gRNA) showed that the dsRNA bands that could be used to discriminate between the dsRNA pattern of the source and the aphid-transmitted isolates were the replicative forms of defective RNAs (D-RNAs). Conversely, the Japanese isolate and two subisolates obtained from it by aphid transmission had the same dsRNA pattern, but one of the subisolates induced milder symptoms in several hosts. Dot-blot hybridization with cDNA probes representing several regions of the gRNA showed that most of the aphid-transmitted isolates differed from the corresponding source isolate by their hybridization pattern. Our results indicate that aphid transmission often sorts the populations of gRNA variants and D-RNAs present in CTV isolates.
引用
收藏
页码:134 / 138
页数:5
相关论文
共 31 条
  • [21] DEFECTIVE RNA MOLECULES ASSOCIATED WITH CITRUS TRISTEZA VIRUS
    MAWASSI, M
    KARASEV, AV
    MIETKIEWSKA, E
    GAFNY, R
    LEE, RF
    DAWSON, WO
    BARJOSEPH, M
    [J]. VIROLOGY, 1995, 208 (01) : 383 - 387
  • [22] IDENTIFICATION OF SPANISH STRAINS OF CITRUS TRISTEZA VIRUS BY ANALYSIS OF DOUBLE-STRANDED-RNA
    MORENO, P
    GUERRI, J
    MUNOZ, N
    [J]. PHYTOPATHOLOGY, 1990, 80 (05) : 477 - 482
  • [23] SEPARATION AND INTERFERENCE OF STRAINS FROM A CITRUS TRISTEZA VIRUS ISOLATE EVIDENCED BY BIOLOGICAL-ACTIVITY AND DOUBLE-STRANDED-RNA (DSRNA) ANALYSIS
    MORENO, P
    GUERRI, J
    BALLESTEROLMOS, JF
    ALBIACH, R
    MARTINEZ, ME
    [J]. PLANT PATHOLOGY, 1993, 42 (01) : 35 - 41
  • [24] MORENO P, 1993, P 12 C INT ORG CITR, P8
  • [25] MORENO P, 1991, P 11 C INT ORG CITR, P20
  • [26] Roistacher C. N., 1991, 11 C INT ORG CITR VI, P7
  • [27] CHARACTERIZATION OF DEFECTIVE INTERFERING RNA COMPONENTS THAT INCREASE SYMPTOM SEVERITY OF BROAD BEAN MOTTLE VIRUS-INFECTIONS
    ROMERO, J
    HUANG, Q
    POGANY, J
    BUJARSKI, JJ
    [J]. VIROLOGY, 1993, 194 (02) : 576 - 584
  • [28] ROUX L, 1991, ADV VIRUS RES, V40, P181
  • [29] Sambrook J., 1989, MOL CLONING
  • [30] The complete genome sequence of the major component of a mild citrus tristeza virus isolate
    Vives, MC
    Rubio, L
    López, C
    Navas-Castillo, J
    Albiach-Martí, MR
    Dawson, WO
    Guerri, J
    Flores, R
    Moreno, P
    [J]. JOURNAL OF GENERAL VIROLOGY, 1999, 80 : 811 - 816