Immobilization of lipase on various acrylic copolymers

被引：47

作者：

Bryjak, J

Bachmann, K

Pawlow, B

Maliszewska, I

Trochimczuk, A

Kolarz, BN

机构：

[1] WROCLAW TECH UNIV,INST ORGAN CHEM BIOCHEM & BIOTECHNOL,PL-50370 WROCLAW,POLAND

[2] WROCLAW TECH UNIV,INST ORGAN & POLYMER TECHNOL,PL-50370 WROCLAW,POLAND

来源：

CHEMICAL ENGINEERING JOURNAL | 1997年 / 65卷 / 03期

关键词：

acrylic carriers; copolymers; lipase; immobilization;

D O I：

10.1016/S1385-8947(97)00021-1

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Polymer carriers with various degrees of hydrophobicity, porosity, and polarity were studied for immobilization of lipase from Candida rugosa. Copolymers and terpolymers were prepared by suspension polymerization of six monomers, ethyl and butyl acrylate, acrylonitrile, methyl methacrylate, 2-hydroxypropyl methacrylate and vinyl acetate, and three crosslinking agents, divinylbenzene, ethylene glycol dimethacrylate and trimethylolpropane triacrylate, in the presence of various diluents. Immobilization of lipase was carried out via glutaraldehyde chemical binding or by adsorption. It was found that the best results were obtained for carriers of butyl acrylate, ethyl acrylate or 2-hydroxypropyl methacrylate crosslinked with ethylene glycol dimethacrylate. (C) 1997 Elsevier Science S.A.

引用

页码：249 / 256

页数：8

共 26 条

[1] STABILITY OF HYDROPHOBIC LIPASE DERIVATIVES IMMOBILIZED ON ORGANIC POLYMER BEADS [J].