Identification of a constitutively active mutant of JAK3 by retroviral expression screening

被引：16

作者：

Choi, Young Lim ^{[1
]}

Kaneda, Ruri ^{[1
]}

Wada, Tomoaki ^{[1
]}

Fujiwara, Shin-ichiro ^{[1
]}

Soda, Manabu ^{[1
]}

Watanabe, Hideki ^{[1
]}

Kurashina, Kentaro ^{[1
]}

Hatanaka, Hisashi ^{[1
]}

Enomoto, Munehiro ^{[1
]}

Takada, Shuji ^{[1
]}

Yamashita, Yoshihiro ^{[1
]}

Mano, Hiroyuki ^{[1
]}

机构：

[1] Jichi Med Univ, Div Funct Genom, Shimotsuke, Tochigi 3290498, Japan

来源：

LEUKEMIA RESEARCH | 2007年 / 31卷 / 02期

关键词：

JAK3; retrovirus; acute myeloid leukemia; cDNA expression library;

D O I：

10.1016/j.leukres.2006.05.006

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To identify transforming genes in acute myeloid leukemia (AML) we here constructed a retroviral cDNA expression library from an AML patient, and then used this library to infect a mouse cell line 32Dcl3-mCAT. cDNA inserts of the cell clones which proliferated in the presence of granulocyte colony-stimulating factor were derived from JAK3 encoding a JAK3 mutant with a valine-to-alanine substitution at codon 674 and two additional amino acid substitutions. The transforming activity of JAK3(V674A) was confirmed by its introduction into 32Dcl3-mCAT. Sequencing of the original JAK3 cDNA derived from the patient, however, failed to detect the V674A mutation. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：203 / 209

页数：7

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